                         SEQUENCE LISTING

<110>  E. I. du Pont de Nemours and Company
 
<120>  Method of Enhancing L-tyrosine Production in Recombinant Bacteria

<130>  CL3286

<160>  40    

<170>  PatentIn version 3.3

<210>  1
<211>  293
<212>  PRT
<213>  Zymomonas mobilis

<400>  1

Met Thr Val Phe Lys His Ile Ala Ile Ile Gly Leu Gly Leu Ile Gly 
1               5                   10                  15      


Ser Ser Ala Ala Arg Ala Thr Lys Ala Tyr Cys Pro Asp Val Thr Val 
            20                  25                  30          


Ser Leu Tyr Asp Lys Ser Glu Phe Val Cys Asp Arg Ala Arg Ala Leu 
        35                  40                  45              


Asn Leu Gly Asp Asn Val Thr Asp Asp Ile Gln Asp Ala Val Arg Glu 
    50                  55                  60                  


Ala Asp Leu Val Leu Leu Cys Val Pro Val Arg Ala Met Gly Ile Val 
65                  70                  75                  80  


Ala Ala Ala Met Ala Pro Ala Leu Lys Lys Asp Val Ile Ile Cys Asp 
                85                  90                  95      


Thr Gly Ser Val Lys Val Ser Val Ile Lys Thr Leu Gln Asp Asn Leu 
            100                 105                 110         


Pro Asn His Ile Ile Val Pro Ser His Pro Leu Ala Gly Thr Glu Asn 
        115                 120                 125             


Asn Gly Pro Asp Ala Gly Phe Ala Glu Leu Phe Gln Asp His Pro Val 
    130                 135                 140                 


Ile Leu Thr Pro Asp Ala His Thr Pro Ala Gln Ala Ile Ala Tyr Ile 
145                 150                 155                 160 


Ala Asp Tyr Trp Glu Glu Ile Gly Gly Arg Ile Asn Leu Met Ser Ala 
                165                 170                 175     


Glu His His Asp His Val Leu Ala Leu Thr Ser His Leu Pro His Val 
            180                 185                 190         


Ile Ala Tyr Gln Leu Ile Gly Met Val Ser Gly Tyr Glu Lys Lys Ser 
        195                 200                 205             


Arg Thr Pro Ile Met Arg Tyr Ser Ala Gly Ser Phe Arg Asp Ala Thr 
    210                 215                 220                 


Arg Val Ala Ala Ser Glu Pro Arg Leu Trp Gln Asp Ile Met Leu Glu 
225                 230                 235                 240 


Asn Ala Pro Ala Leu Leu Pro Val Leu Asp His Phe Ile Ala Asp Leu 
                245                 250                 255     


Lys Lys Leu Arg Thr Ala Ile Ala Ser Gln Asp Glu Asp Tyr Leu Leu 
            260                 265                 270         


Glu His Phe Lys Glu Ser Gln Lys Ala Arg Leu Ala Leu Lys Thr Asp 
        275                 280                 285             


His Asp Ile His Pro 
    290             


<210>  2
<211>  882
<212>  DNA
<213>  Zymomonas mobilis

<400>  2
atgaccgtct ttaagcatat tgccattatc ggattaggac tgatcggttc ctctgcggca     60

cgggcaacaa aggcctattg tcctgatgta acggtcagtc tctatgacaa aagcgaattt    120

gtctgcgaca gagctagagc gctcaatctc ggcgacaatg tcaccgatga tattcaagat    180

gcggttcgtg aggctgatct ggtgctatta tgcgtgccag tcagggcaat gggtatcgtc    240

gcggcagcga tggcaccggc gctgaaaaaa gacgttatta tctgcgatac aggttcggta    300

aaagtcagcg ttataaaaac gctgcaagac aatttaccca atcacattat tgttcctagc    360

catcctttgg ctgggactga aaataacgga cccgacgccg gttttgctga attattccaa    420

gaccatcctg ttattttgac ccccgatgcc catacaccgg cacaggctat cgcctatatc    480

gccgattatt gggaagaaat tggtgggcgt atcaatctga tgtcggcgga acatcacgat    540

cacgttttag cgcttaccag ccatttgcct catgtcattg cataccaact tatagggatg    600

gtatcgggtt atgagaaaaa aagccggaca cccatcatgc gttattcggc aggcagcttt    660

cgggatgcga cgcgggtagc ggcttcggaa cctcgtctct ggcaagatat tatgctggaa    720

aatgcgcctg ctcttttacc agtgctggat cattttatcg cagatctcaa aaaattgcgg    780

acagctattg cttcgcaaga tgaggattat cttcttgagc atttcaaaga atcgcagaaa    840

gcgcgtttag ccttaaaaac agaccacgat attcaccctt aa                       882


<210>  3
<211>  140
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  3
tgaagaagag tagtccttta tattgagtgt atcgccaacg cgccttcggg cgcgtttttt     60

tgaaaaggtg ccggatgatg tgaatcatcc ggcactggat tattactggc gattgtcatt    120

ttaagaccca ctttcacatt                                                140


<210>  4
<211>  140
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  4
aatgacaatc gccagtaata atccagtgcc ggatgattca catcatccgg caccttttca     60

aaaaaacgcg cccgaaggcg cgttggcgat acactcaata taaaggacta ctcttcttca    120

ctaagcactt gtctcctgtt                                                140


<210>  5
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  5
ttaagaccca ctttcacatt                                                 20


<210>  6
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  6
ctaagcactt gtctcctgtt                                                 20


<210>  7
<211>  66
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  7
aattcatcag gatctgaacg ggcagctgac ggctcgcgtg gcttaacgtc ttgagcgatt     60

gtgtag                                                                66


<210>  8
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  8
aaaacattat ccagaacggg agtgcgcctt gagcgacacg aatatgaata tcctccttag     60

ttcc                                                                  64


<210>  9
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  9
ctaaggagga tattcatatt cgtgtcgctc aaggcgcact                           40


<210>  10
<211>  66
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  10
cgacttcatc aatttgatcg cgtaatgcgg tcaattcagc aaccatggtc tgtttcctgt     60

gtgaaa                                                                66


<210>  11
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  11
caaccgcgca gtgaaatgaa atacgg                                          26


<210>  12
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  12
gcgctccgga acataaatag gcagtc                                          26


<210>  13
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  13
cacctgatga ccgtctttaa gcatatt                                         27


<210>  14
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  14
ttaagggcga atatcgtggt                                                 20


<210>  15
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  15
cacctgatgg ttgctgaatt gaccgc                                          26


<210>  16
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  16
ttactggcga ttgtcattcg                                                 20


<210>  17
<211>  1122
<212>  DNA
<213>  E. coli

<400>  17
atggttgctg aattgaccgc attacgcgat caaattgatg aagtcgataa agcgctgctg     60

aatttattag cgaagcgtct ggaactggtt gctgaagtgg gcgaggtgaa aagccgcttt    120

ggactgccta tttatgttcc ggagcgcgag gcatctatgt tggcctcgcg tcgtgcagag    180

gcggaagctc tgggtgtacc gccagatctg attgaggatg ttttgcgtcg ggtgatgcgt    240

gaatcttact ccagtgaaaa cgacaaagga tttaaaacac tttgtccgtc actgcgtccg    300

gtggttatcg tcggcggtgg cggtcagatg ggacgcctgt tcgagaagat gctgaccctc    360

tcgggttatc aggtgcggat tctggagcaa catgactggg atcgagcggc tgatattgtt    420

gccgatgccg gaatggtgat tgttagtgtg ccaatccacg ttactgagca agttattggc    480

aaattaccgc ctttaccgaa agattgtatt ctggtcgatc tggcatcagt gaaaaatggg    540

ccattacagg ccatgctggt ggcgcatgat ggtccggtgc tggggctaca cccgatgttc    600

ggtccggaca gcggtagcct ggcaaagcaa gttgtggtct ggtgtgatgg acgtaaaccg    660

gaagcatacc aatggtttct ggagcaaatt caggtctggg gcgctcggct gcatcgtatt    720

agcgccgtcg agcacgatca gaatatggcg tttattcagg cactgcgcca ctttgctact    780

tttgcttacg ggctgcacct ggcagaagaa aatgttcagc ttgagcaact tctggcgctc    840

tcttcgccga tttaccgcct tgagctggcg atggtcgggc gactgtttgc tcaggatccg    900

cagctttatg ccgacatcat tatgtcgtca gagcgtaatc tggcgttaat caaacgttac    960

tataagcgtt tcggcgaggc gattgagttg ctggagcagg gcgataagca ggcgtttatt   1020

gacagtttcc gcaaggtgga gcactggttc ggcgattacg cacagcgttt tcagagtgaa   1080

agccgcgtgt tattgcgtca ggcgaatgac aatcgccagt aa                      1122


<210>  18
<211>  373
<212>  PRT
<213>  E. coli

<400>  18

Met Val Ala Glu Leu Thr Ala Leu Arg Asp Gln Ile Asp Glu Val Asp 
1               5                   10                  15      


Lys Ala Leu Leu Asn Leu Leu Ala Lys Arg Leu Glu Leu Val Ala Glu 
            20                  25                  30          


Val Gly Glu Val Lys Ser Arg Phe Gly Leu Pro Ile Tyr Val Pro Glu 
        35                  40                  45              


Arg Glu Ala Ser Met Leu Ala Ser Arg Arg Ala Glu Ala Glu Ala Leu 
    50                  55                  60                  


Gly Val Pro Pro Asp Leu Ile Glu Asp Val Leu Arg Arg Val Met Arg 
65                  70                  75                  80  


Glu Ser Tyr Ser Ser Glu Asn Asp Lys Gly Phe Lys Thr Leu Cys Pro 
                85                  90                  95      


Ser Leu Arg Pro Val Val Ile Val Gly Gly Gly Gly Gln Met Gly Arg 
            100                 105                 110         


Leu Phe Glu Lys Met Leu Thr Leu Ser Gly Tyr Gln Val Arg Ile Leu 
        115                 120                 125             


Glu Gln His Asp Trp Asp Arg Ala Ala Asp Ile Val Ala Asp Ala Gly 
    130                 135                 140                 


Met Val Ile Val Ser Val Pro Ile His Val Thr Glu Gln Val Ile Gly 
145                 150                 155                 160 


Lys Leu Pro Pro Leu Pro Lys Asp Cys Ile Leu Val Asp Leu Ala Ser 
                165                 170                 175     


Val Lys Asn Gly Pro Leu Gln Ala Met Leu Val Ala His Asp Gly Pro 
            180                 185                 190         


Val Leu Gly Leu His Pro Met Phe Gly Pro Asp Ser Gly Ser Leu Ala 
        195                 200                 205             


Lys Gln Val Val Val Trp Cys Asp Gly Arg Lys Pro Glu Ala Tyr Gln 
    210                 215                 220                 


Trp Phe Leu Glu Gln Ile Gln Val Trp Gly Ala Arg Leu His Arg Ile 
225                 230                 235                 240 


Ser Ala Val Glu His Asp Gln Asn Met Ala Phe Ile Gln Ala Leu Arg 
                245                 250                 255     


His Phe Ala Thr Phe Ala Tyr Gly Leu His Leu Ala Glu Glu Asn Val 
            260                 265                 270         


Gln Leu Glu Gln Leu Leu Ala Leu Ser Ser Pro Ile Tyr Arg Leu Glu 
        275                 280                 285             


Leu Ala Met Val Gly Arg Leu Phe Ala Gln Asp Pro Gln Leu Tyr Ala 
    290                 295                 300                 


Asp Ile Ile Met Ser Ser Glu Arg Asn Leu Ala Leu Ile Lys Arg Tyr 
305                 310                 315                 320 


Tyr Lys Arg Phe Gly Glu Ala Ile Glu Leu Leu Glu Gln Gly Asp Lys 
                325                 330                 335     


Gln Ala Phe Ile Asp Ser Phe Arg Lys Val Glu His Trp Phe Gly Asp 
            340                 345                 350         


Tyr Ala Gln Arg Phe Gln Ser Glu Ser Arg Val Leu Leu Arg Gln Ala 
        355                 360                 365             


Asn Asp Asn Arg Gln 
    370             


<210>  19
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  19
gagcgcgagg catctatttt ggcctcgcg                                       29


<210>  20
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  20
cgcgaggcca aaatagatgc ctcgcgctc                                       29


<210>  21
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  21
ccctctcggg ttatcgggtg cggattctg                                       29


<210>  22
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  22
cagaatccgc acccgataac ccgagaggg                                       29


<210>  23
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  23
gctacttttg ctcacgggct gcacctggc                                       29


<210>  24
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  24
gccaggtgca gcccgtgagc aaaagtagc                                       29


<210>  25
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  25
ctggttcggc gattacgtac agcgttttca gagtg                                35


<210>  26
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  26
cactctgaaa acgctgtacg taatcgccga accag                                35


<210>  27
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  27
ggagcgcgag gcatctatgt tggcctcgc                                       29


<210>  28
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  28
gcgaggccaa catagatgcc tcgcgctcc                                       29


<210>  29
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  29
cactttgtcc gtcactg                                                    17


<210>  30
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  30
cagtgacgga caaagtg                                                    17


<210>  31
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  31
ggcgtttatt caggcac                                                    17


<210>  32
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  32
gtgcctgaat aaacgcc                                                    17


<210>  33
<211>  17
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  33
gtaaaacgac ggccagt                                                    17


<210>  34
<211>  16
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  34
aacagctatg accatg                                                     16


<210>  35
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  35
cacctgatgg gcgatattat gttt                                            24


<210>  36
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  36
ttattttttc tgatccag                                                   18


<210>  37
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  37
cacctgatga acagcgcgcc gatgt                                           25


<210>  38
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  38
ttattccgcg cctttgcc                                                   18


<210>  39
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  39
cacctgatga ccaccgcgcc gagc                                            24


<210>  40
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  40
ttacgcctgt ttcgcttc                                                   18


